ZEISS Microscopy developed a new lightsheet microscope system for observing the differentiation of cell groups or the development of whole organisms over days without compromising the sample. The light sheet microscope delivers more information than established fluorescence microscopy processes.
The developed Lightsheet Z.1 system uses a new, unique lens design, which combines a cylindrical lens with laser scanning. One of the scanner modules used was developed by the Fraunhofer IPMS and CTR research centre. Starting with the IPM’s micro scanning mirror, CTR created a miniaturised platform (scanner module) for operating, controlling and packaging micro scanning mirrors with an oscillating axle. The microscanner together with the electronics was adapted to meet the microscope system’s demands. It is small in size and features a high scanning frequency with a highly stable and extremely low-noise form of oscillations. The micro scanning mirror is the core component. The scanner module with integrated micro scanning mirror, the electronics required and packaging is a modular design. Once adapted, the scanner module was integrated in the ZEISS Lightsheet Z.1 microscope.
Optical design, system design simulations, electronics and developing a prototype.